Journal: medRxiv

Article Title: Polymorphism in IFNAR contributes to glucocorticoid response and outcome in ARDS and COVID-19

doi: 10.1101/2022.03.10.22272123

Figure Lengend Snippet: (A ) STAT1 expression in the lung after 4-day culture in the presence of IFN beta with or without hydrocortisone (HC). ( B) pSTAT1 expression in the same specimens as in A. ( C) Example photomicrographs showing higher STAT2 expression in a TT patient than in a CT patient and the effect of HC on its nuclear translocation. Most STAT2 remains in the cytoplasm of the CT patients, whereas nuclear expression is prominent in the TT patient. Indicated insets are shown in the bottom row. Arrows. ( D ) Combined results of all patients noting that two CT samples are excluded in the data as the patients were already under glucocorticoid treatment at the time of sample acquisition. Ns, not significant; *P<0.05; **P<0.01; and ***P<0.001

Article Snippet: The first stage antibodies were anti-alpha chain of the IFN alpha/beta receptor (St John’s Laboratory STJ112765) that was used 1:2000 and 1:5000 and anti-Stat1 (1:400, 9175S), anti-pStat1(1:100, 9167S) and anti-Stat2 (1:200, 72604S) all from Cell Signalling.

Techniques: Expressing, Translocation Assay

Journal: Acta Oncologica

Article Title: Identification of a novel resistance mechanism in venetoclax treatment and its prediction in chronic lymphocytic leukemia

doi: 10.1080/0284186x.2021.1878388

Figure Lengend Snippet: Figure 2. Change of protein expression during venetoclax treatment measured by apoptosis arrays.

Article Snippet: Apoptosis array (R&D Systems Minneapolis, USA, Human apoptosis antibody array kit) was performed from the mononuclear cell fraction of the samples according to the manufacturer’s instructions (Analysis ImageJ software version 1.50d, NIH, USA) [10,11].

Techniques: Expressing

Journal: iScience

Article Title: Loss of histone reader Phf7 leads to immune pathways activation via endogenous retroviruses during spermiogenesis

doi: 10.1016/j.isci.2023.108030

Figure Lengend Snippet:

Article Snippet: Rabbit Lamin B1 Polyclonal , bioss , bs-1840R.

Techniques: ROS Assay, TUNEL Assay, Apoptosis Assay, Bicinchoninic Acid Protein Assay, Recombinant, SYBR Green Assay, Gene Knockout, Software